Engineered axon tracts within tubular biohybrid scaffolds

Injuries to the nervous system that involve disruption of axonal pathways are devastating individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy overcome obstacles limiting axon regenerationin vivo, based on combination hyaluronic acid (HA) single-channel tubular conduit filled with poly-L-lactide (PLA) fibres in its lumen, pre-cultured Schwann cells (SCs) as supportive extension. The HA PLA sustain proliferation SC, which enhance growth acting feeder layer factor pumps. parallel unidirectional ensemble formed by SC tries recapitulate directional features system. A dorsal root ganglion (DRG) explant is planted one conduit's ends follow outgrowth from DRG. After 21 d co-culture DRG + SC-seeded ensemble, extension throughout scanning, transmission electronic confocal microscopy, order study grown axons their association. separate effects also experimentally addressed. biohybrid thus produced may be considered synthetic pathway, results could use strategies for regeneration tracts.